TY - JOUR
T1 - Voltage-Gated Potassium+ Channel Expression in Coronary Artery Smooth Muscle Cells of SHR and WKY
AU - Hu, Zhi
AU - Ma, Aiqun
AU - Zhang, Yushun
AU - Xi, Yutao
AU - Fan, Lihong
AU - Wang, Tingzhong
AU - Zhang, Tingting
N1 - Publisher Copyright:
© 2014, Springer Science+Business Media New York.
PY - 2014/12
Y1 - 2014/12
N2 - This study aims to compare the expression of genes and the molecular characteristic of voltage-gated K+ channels, which make great effort in maintaining and controlling smooth muscle contraction, cellular membrane potential, and intracellular calcium ion currents in artery smooth muscle cells of SHR and WKY. Expression of potassium ions family in coronary artery was detected through reverse transcription polymerase chain reaction quantitatively. Significant levels of voltage-gated K+ channels α1.2, α1.5, and β1.1 expression were all proved to be significantly higher in smooth muscles of SHR than WKY. Whole-cell voltage-gated K+ channel currents were larger in SHR artery smooth muscles than the ones of WKY. Moreover, the voltage dependence of voltage-gated potassium channel activation was more negative in artery smooth muscle of SHR than that of WKY, while voltage dependence of availability was not different. The above diversity of voltage-gated potassium channel detected in gene expression and electrical character in coronary artery smooth muscle of SHR than that of WKY might be an underling mechanism associated with the membrane potential depolarization in artery smooth muscle of SHR.
AB - This study aims to compare the expression of genes and the molecular characteristic of voltage-gated K+ channels, which make great effort in maintaining and controlling smooth muscle contraction, cellular membrane potential, and intracellular calcium ion currents in artery smooth muscle cells of SHR and WKY. Expression of potassium ions family in coronary artery was detected through reverse transcription polymerase chain reaction quantitatively. Significant levels of voltage-gated K+ channels α1.2, α1.5, and β1.1 expression were all proved to be significantly higher in smooth muscles of SHR than WKY. Whole-cell voltage-gated K+ channel currents were larger in SHR artery smooth muscles than the ones of WKY. Moreover, the voltage dependence of voltage-gated potassium channel activation was more negative in artery smooth muscle of SHR than that of WKY, while voltage dependence of availability was not different. The above diversity of voltage-gated potassium channel detected in gene expression and electrical character in coronary artery smooth muscle of SHR than that of WKY might be an underling mechanism associated with the membrane potential depolarization in artery smooth muscle of SHR.
KW - Artery
KW - Hypertension
KW - Voltage-gated K channels
UR - https://www.scopus.com/pages/publications/84939886524
U2 - 10.1007/s12013-014-0120-4
DO - 10.1007/s12013-014-0120-4
M3 - 文章
C2 - 25030407
AN - SCOPUS:84939886524
SN - 1085-9195
VL - 70
SP - 1725
EP - 1731
JO - Cell Biochemistry and Biophysics
JF - Cell Biochemistry and Biophysics
IS - 3
ER -