Visual detection of HPV16 using a photoactivatable CRISPR-Cas12 system

Xiaoya Gu; Zhe Ma; Lin Zhou; Na Li; Shijiang Yu; Fu Wang; Ruifang An

doi:10.1039/d5cc00369e

Visual detection of HPV16 using a photoactivatable CRISPR-Cas12 system

Xiaoya Gu
, Zhe Ma
, Lin Zhou
, Na Li
, Shijiang Yu
, Fu Wang
, Ruifang An

医学部

The First Affiliated Hospital of Xi’an Jiaotong University
Xi'an Jiaotong University
The Second Affiliated Hospital of Xi'an Jiaotong University

科研成果: 期刊稿件 › 文章 › 同行评审

7 引用（Scopus）

摘要

Human papillomavirus (HPV) screening is crucial for the diagnosis of cervical cancer. In this study, we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm. It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.

源语言	英语
页（从-至）	4383-4386
页数	4
期刊	Chemical Communications
卷	61
期	22
DOI	https://doi.org/10.1039/d5cc00369e
出版状态	已出版 - 18 2月 2025

联合国可持续发展目标

此成果有助于实现下列可持续发展目标：

可持续发展目标 3 良好健康与福祉

访问文件

10.1039/d5cc00369e

其它文件与链接

链接到 Scopus 的出版物

引用此

@article{fc72b7d922b34a4d865afae294ac6a7d,

title = "Visual detection of HPV16 using a photoactivatable CRISPR-Cas12 system",

abstract = "Human papillomavirus (HPV) screening is crucial for the diagnosis of cervical cancer. In this study, we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm. It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.",

author = "Xiaoya Gu and Zhe Ma and Lin Zhou and Na Li and Shijiang Yu and Fu Wang and Ruifang An",

note = "Publisher Copyright: {\textcopyright} 2025 The Royal Society of Chemistry.",

year = "2025",

month = feb,

day = "18",

doi = "10.1039/d5cc00369e",

language = "英语",

volume = "61",

pages = "4383--4386",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "22",

}

TY - JOUR

T1 - Visual detection of HPV16 using a photoactivatable CRISPR-Cas12 system

AU - Gu, Xiaoya

AU - Ma, Zhe

AU - Zhou, Lin

AU - Li, Na

AU - Yu, Shijiang

AU - Wang, Fu

AU - An, Ruifang

PY - 2025/2/18

Y1 - 2025/2/18

N2 - Human papillomavirus (HPV) screening is crucial for the diagnosis of cervical cancer. In this study, we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm. It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.

AB - Human papillomavirus (HPV) screening is crucial for the diagnosis of cervical cancer. In this study, we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm. It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.

UR - https://www.scopus.com/pages/publications/85219075321

U2 - 10.1039/d5cc00369e

DO - 10.1039/d5cc00369e

M3 - 文章

C2 - 39989356

AN - SCOPUS:85219075321

SN - 1359-7345

VL - 61

SP - 4383

EP - 4386

JO - Chemical Communications

JF - Chemical Communications

IS - 22

ER -

Visual detection of HPV16 using a photoactivatable CRISPR-Cas12 system

摘要

联合国可持续发展目标

访问文件

其它文件与链接

学术指纹

引用此