VEGF antisense oligodeoxynucleotides in enhancing the apoptosis-inducing effect of Cinobufotalin on K562 cells

Objective: To study the vascular endothelium growth factor (VEGF) antisense oligonucleotide (ASON) Cinobufotalin in enhancing apoptosis induced by human chronic myeloid leukemia K562 cell line. Methods The synthesis of VEGF ASON was transfected into the K562 cells; Cinobufotalin of four concentrations (1, 3, 5 and 7 mg/L) acted on the K562 cell line for 24 h, 48 h and 72 h. Western blot was used to detect VEGF protein expression, while in situ apoptosis (TUNED and flow cytometry method (FCM method) were employed to detect the apoptosis. Results The different doses of Cinobufotalin all inhibited K562 cell line in time- and dose- dependent manners. K562 cell line transfected by VEGF ASON had a more pronounced induction of apoptosis. Conclusion The in vitro Cinobufotalin at a certain range of concentration can induce apoptosis in K562 cell line, and VEGF ASON enhances Cinobufotalin's effect in inducing apoptosis of K562 cells.