VASH2在胃癌细胞上皮间质转化中的作用

Objective To investigate the role of VASH2 gene expression in the process of epithelial-mesenchymal transition of gastric cancer cells so as to elucidate its mechanism in gastric carcinogenesis, invasion and metastasis. Methods The effects of VASH2 expression on the proliferation, invasion and migration of gastric cancer cell line were observed. The effect of VASH2 expression on the epithelial-mesenchymal transition of gastric cancer cells was analyzed by immunoblotting. The overall survival of different VASH2 expressions was analyzed using TCGA database. Results The invasion and migration abilities of the cells were significantly decreased compared with those in the control group after VASH2 knockout (both P<0.05). The expression of E-cadherin was significantly up-regulated after VASH2 knockout, while those of Vimentin and Snail were significantly decreased (all P<0.05). The 5-year overall survival was significantly decreased in patients with high expression of VASH2. Conclusion VASH2 is a regulator of gastric cancer cells for cell proliferation, migration and invasion abilities. The invasion and metastasis of gastric cancer cell lines are inhibited by VASH2 knock-out via EMT inhibition.