@inproceedings{dd78860c6ed146b2b63a7f21237fc3ae,
title = "Ureolysis and denitrification based microbial strategies for self-healing concrete",
abstract = "Ureolysis and denitrification were examined for microbially induced calcium carbonate precipitation and concrete self-healing. In a first set of experiments, the immobilization of Bacillus sphaericus, an ureolytic strain, with Diatomaceous Earth (DE) and its crack healing efficiency were investigated. The ureolytic activity of DE immobilized bacteria was found to be much higher than that of non-immobilized bacteria (12-17 g/L urea degradation and 1 g/L urea degradation in 3 days, respectively) in cement slurry. Cracks with a width ranging from 0.15 to 0.17mm in the specimens containing DE immobilized bacteria were completely healed within 40 days. The specimens having bacteria showed 70\% less water absorption than the reference specimens after healing. Denitrifiers (9 unique strains) were selected from soil and exposed to dehydration stress in minimal medium. The capability of denitrifiers to re-activate after dehydration and precipitate CaCO3 in a minimal medium enriched with nitrate (NO3) and calcium (Ca2+) ionswere tested. A calcium carbonate (CaCO3) precipitation yield of 4.8-6.7 g CaCO3/g NO3 was obtained.",
keywords = "Crack, Denitrification, Self-healing",
author = "Ersan, \{Y. C.\} and Wang, \{J. Y.\} and N. Boon and \{De Belie\}, N.",
year = "2014",
doi = "10.1201/b17394-10",
language = "英语",
isbn = "9781138027084",
series = "Concrete Solutions - Proceedings of Concrete Solutions, 5th International Conference on Concrete Repair",
publisher = "CRC Press/Balkema",
pages = "59--64",
booktitle = "Concrete Solutions - Proceedings of Concrete Solutions, 5th International Conference on Concrete Repair",
note = "5th International Conference on Concrete Repair ; Conference date: 01-09-2014 Through 03-09-2014",
}