TY - JOUR
T1 - Treatment of ribonucleoside solution with atmospheric-pressure plasma
AU - Zhang, Xianhui
AU - Zhou, Renwu
AU - Zhou, Rusen
AU - Chen, Maodong
AU - Li, Jiangwei
AU - Sun, Yue
AU - Chen, Qiang
AU - Yang, Size
AU - Liu, Qing Huo
N1 - Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - A self-made microplasma jet array device is used to treat deoxyadenosine (dA) and adenosine (Ado) solutions to avoid the effect of air on genetic materials. Results show that Ado is easily converted into dA through deoxidation reaction. In addition, dA solution is treated with different microplasma arrays (i.e., atmospheric-pressure Ar, N2, air, and O2) for comparative analysis. The dA molecule is relatively stable within the Ar plasma for 3 min treatment time. This molecule can be modified into different structures with N2, air, and O2 microplasma arrays. The results obtained in this study provide references for the application of plasma in biology and the effect of oxygen-containing free radicals in biological plasma. As an immediate and latest area of research focus, atmospheric microplasma has wide applications in the biomedical field. However, effects of plasma on ribonucleoside in DNA are less studied and reported. A self-made microplasma jet array device is used to treat basic blocks of DNA, and online ion-pair reversed-phase liquid chromatography (IP-RPLC) with electrospray tandem mass spectrometry (ESI-MS) is used to detect the reaction process. Results show oxidative damage to DNA is a complex process, influenced by charge transport and reactions that are controlled by a combination of enthalpic, entropic, steric, and compositional factors.
AB - A self-made microplasma jet array device is used to treat deoxyadenosine (dA) and adenosine (Ado) solutions to avoid the effect of air on genetic materials. Results show that Ado is easily converted into dA through deoxidation reaction. In addition, dA solution is treated with different microplasma arrays (i.e., atmospheric-pressure Ar, N2, air, and O2) for comparative analysis. The dA molecule is relatively stable within the Ar plasma for 3 min treatment time. This molecule can be modified into different structures with N2, air, and O2 microplasma arrays. The results obtained in this study provide references for the application of plasma in biology and the effect of oxygen-containing free radicals in biological plasma. As an immediate and latest area of research focus, atmospheric microplasma has wide applications in the biomedical field. However, effects of plasma on ribonucleoside in DNA are less studied and reported. A self-made microplasma jet array device is used to treat basic blocks of DNA, and online ion-pair reversed-phase liquid chromatography (IP-RPLC) with electrospray tandem mass spectrometry (ESI-MS) is used to detect the reaction process. Results show oxidative damage to DNA is a complex process, influenced by charge transport and reactions that are controlled by a combination of enthalpic, entropic, steric, and compositional factors.
KW - adenosine(Ado) oxidization
KW - deoxyadenosine(dA)
KW - electrospray tandem mass spectrometry (ESI-MS)
KW - microplasma
KW - online ion-pair reversed-phase liquid chromatography (IP-RPLC)
UR - https://www.scopus.com/pages/publications/84963626433
U2 - 10.1002/ppap.201500088
DO - 10.1002/ppap.201500088
M3 - 文章
AN - SCOPUS:84963626433
SN - 1612-8850
VL - 13
SP - 429
EP - 437
JO - Plasma Processes and Polymers
JF - Plasma Processes and Polymers
IS - 4
ER -