TY - JOUR
T1 - Treatment effects of 3D-printed PCL/Fe3O4@ ZIF-8 magnetic nanocomposite on infected bone defect
AU - Xiao, Yun
AU - Ding, Yi
AU - Qiu, Jun
AU - Zhang, Xiaonan
AU - Zheng, Yanzhen
AU - Huang, Chong
AU - Zhao, Lu
AU - Tang, Zihao
AU - Chen, Yuanli
AU - Liu, Yiwen
AU - Zhao, Kezhen
AU - Guo, Kai
AU - Jing, Li
AU - Ding, Mingchao
AU - Zong, Chunlin
AU - He, Jiankang
AU - Tian, Lei
N1 - Publisher Copyright:
© 2024 Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution, and reproduction in any medium, provided the original work is properly cited.
PY - 2024
Y1 - 2024
N2 - Treatment of large bone defects remains a clinical challenge, especially for defects compounded by infection. It is essential to develop dual functional therapeutic systems that inhibit bacterial growth and promote bone regeneration for infected bone defects treatment. However, the ideal bone substitute biomaterials to repair infected bone defects remain scarce. In this study, we fabricated a novel magnetic nanocomposite with polycaprolactone (PCL)/Fe3O4@zinc-based imidazole zeolite framework-8 (ZIF-8) through three-dimensional (3D) printing technology. The biomaterial characterization, biocompatibility, antibacterial activity, and osteogenic ability of the 3D-printed PCL/Fe3O4@ZIF-8 nanocomposite were systematically investigated in vivo and in vitro. The 3D-printed PCL/Fe3O4@ZIF-8 nanocomposite scaffolds showed a square porous grid structure with rough surface, thermal stability, superparamagnetic character, and slow release of Zn2+. The elevation of Fe3O4@ZIF-8 concentration increased surface roughness and porosity, improved the mechanical properties, and enhanced the saturation magnetization of PCL/Fe3O4@ZIF-8 scaffolds. The PCL/Fe3O4@ZIF-8 scaffolds possessed good biocompatibility and promoted the proliferation and adhesion of rat bone marrow mesenchymal stem cells (BMSCs). The PCL/Fe3O4@ZIF-8 scaffolds also upregulated the expression of osteogenic-related genes and proteins and promoted the osteogenic differentiation of BMSCs by activating the Wnt/β-catenin signaling pathway. Furthermore, the scaffolds showed excellent antibacterial activities, which increased with increasing Fe3O4@ ZIF-8 nanoparticle concentration. In vivo experiments proved that the scaffolds eliminated infection and promoted new bone formation in infected bone defect. Given excellent osteogenic and antibacterial activities, the 3D-printed PCL/Fe3O4@ZIF-8 nanocomposite scaffolds could serve as novel materials for the treatment of infected bone defects.
AB - Treatment of large bone defects remains a clinical challenge, especially for defects compounded by infection. It is essential to develop dual functional therapeutic systems that inhibit bacterial growth and promote bone regeneration for infected bone defects treatment. However, the ideal bone substitute biomaterials to repair infected bone defects remain scarce. In this study, we fabricated a novel magnetic nanocomposite with polycaprolactone (PCL)/Fe3O4@zinc-based imidazole zeolite framework-8 (ZIF-8) through three-dimensional (3D) printing technology. The biomaterial characterization, biocompatibility, antibacterial activity, and osteogenic ability of the 3D-printed PCL/Fe3O4@ZIF-8 nanocomposite were systematically investigated in vivo and in vitro. The 3D-printed PCL/Fe3O4@ZIF-8 nanocomposite scaffolds showed a square porous grid structure with rough surface, thermal stability, superparamagnetic character, and slow release of Zn2+. The elevation of Fe3O4@ZIF-8 concentration increased surface roughness and porosity, improved the mechanical properties, and enhanced the saturation magnetization of PCL/Fe3O4@ZIF-8 scaffolds. The PCL/Fe3O4@ZIF-8 scaffolds possessed good biocompatibility and promoted the proliferation and adhesion of rat bone marrow mesenchymal stem cells (BMSCs). The PCL/Fe3O4@ZIF-8 scaffolds also upregulated the expression of osteogenic-related genes and proteins and promoted the osteogenic differentiation of BMSCs by activating the Wnt/β-catenin signaling pathway. Furthermore, the scaffolds showed excellent antibacterial activities, which increased with increasing Fe3O4@ ZIF-8 nanoparticle concentration. In vivo experiments proved that the scaffolds eliminated infection and promoted new bone formation in infected bone defect. Given excellent osteogenic and antibacterial activities, the 3D-printed PCL/Fe3O4@ZIF-8 nanocomposite scaffolds could serve as novel materials for the treatment of infected bone defects.
KW - 3D printing
KW - Antibacterial activity
KW - Bone regeneration
KW - FeO nanoparticles
KW - Infected bone defect
KW - Zinc-based imidazole zeolite framework-8
UR - https://www.scopus.com/pages/publications/85203262382
U2 - 10.36922/ijb.2271
DO - 10.36922/ijb.2271
M3 - 文章
AN - SCOPUS:85203262382
SN - 2424-8002
VL - 10
SP - 297
EP - 318
JO - International Journal of Bioprinting
JF - International Journal of Bioprinting
IS - 4
M1 - 2271
ER -