The experimental study of resveratrol in the acute pancreatitis combined with lung injury in rats

Objective: To explore the relationship of acute lung injury (ALI) in experimental pancreatitis to selectin P and selectin E and to study the effects of resveratrol on ALI. Methods: A total of 96 male SD rats were divided into 4 groups radomly with 24 rats in each group: control group, pancreatitis group (model), resveratrol-treated group and tween80-treated group (solvent). The model of acute pancreatitis was performed by injecting 4% sodium taurocholate into the biliopancreatic duct. Resveratrol was given through penis vein after modeling. Tween80 was used as a solvent of resveratrol. The rats were killed at 3, 6, 12 hours after modeling. The degree of ALI was judged by studying the pulmonary morphologic changes, leucocytes of bronchi alveolar lung fluid (BALF) and myeloperoxidase (MPO) in lung tissue. The selectin P and selectin E in vascular endothelium of lung tissue was detected immunohistologically. Results: Severe pulmonary morphologic changes were observed in pancreatitis group. The counts of leucocytes in BALF and the amount of MPO were increased with time passing. There was a less degree of ALI in resveratrol-treated rats than in pancreatitis rats. Resveratrol reduced the counts of leucocytes in BALF and the amount of MPO(P<0.05). Selectin P and selectin E are expressed more positive in pancreatitis group than in treatment group(P<0.05). Conclusion: The effect of reduced severity of ALI during acute pancreatitis by resveratrol might be related to the inhibition of the selectin expression and reduction of the adhension between inflammatory cells and endothelium cells.