The establishment and identification of GP_x-1^p198L gene systemic expression transgenic mice

Objective: To generate systemic expression human cellular glutathione peroxidase-1 (GP_x-1) (198Leu) transgenic mice model in order to investigate the functional variants in GP_x-1 gene in oxidative stressrelated diseases. Methods: After linearization with BamH I and Acc I, the transgenic construct GP_x-1 (198Leu) was microinjected into the zygotes of C57BL/6J mice to generate transgenic mice, whose genotype was detected by PCR with specific primers. The GP_x-1 gene expression profile was determined by Western blotting. Results: 13 transgenic founder mice were successfully generated. Western blotting result showed that the protein expression level of 4 transgenic mice in hearts were higher than that of wild type mice. Conclusion: Human GP_x-1^P198L transgenic mice was successfully established. This kind of animal model is of significance for making further researches on oxidative stress-related diseases.