The application of STR loci genescan in paternity testing

Objective: To search for an easy standardization paternity testing method. Methods: Based on nine completed data and stable tetranucleotide STR loci, 45 paternity cases were typed by using fluorescence labeling primer, multiplex PCR, higher sensitivity 377 DNA sequencer, and then the computer automatically collected the data and made analysis, STR loci alleles and genotypes were determined. Results: There was no relationship between alleged fathers and children in 21 cases. But in 24 cases the relationship could not be excluded. Conclusion: The STR loci genescan method is convenient, time-saving, fast and has higher sensitivity. The results are accurate and reliable.