Study on invasion-related factors of three human urinary bladder cancer cell lines

Objective: To investigate the various mechanisms of invasion in urinary bladder cancer. Methods: The cloning efficiency and activity of adhesion of basement membrane were analysed. The fluidity of the membrane and the expression of E-cadherin, and MMP-2, and CD44 were carried out by fluorescence polarization, immunohistochemical staining and flow cytometry. Results: The cloning efficiency of BLX and BLZ was 6.0% and 3.0% in soft agar, respectively. The colony of BLS was not formed in soft agar. The fluidity of the membrane was different in the three cell lines. The expressions of E-cadherin and CD44 in BLS and BLZ were greater than that in BLX cell line. Significant difference in MMP-2 was not found in the three cell lines. Conclusion: The research suggests that difference in fluidity of the membrane and expression of CD44 may play an important role in invasion of urinary bladder cancer.