Study on estimation of radiation dose using FTIR in mice thymus

Fourier transform infrared spectroscopy (FTIR) was applied to study the biochemical changes in the radiation damaged mouse thymus which increased with radiation dose and provided a new method for the estimation of the radiation dose of radiation damaged patients. The results demonstrated that with the dose increasing, the peak positions like 1550, 1400, 1400 and 1640 cm^-1 at the dose of 2, 3 and 5 Gy showed some difference, and there was obvious variance in the intensity: (1) The intensity ratio of 1085 to 1236 cm^-1 related to nucleic acid tended to decrease. (2) The intensity ratio of 1640/1550 decreased. (3) The intensity at 2958, 2925, 1460 and 1400 cm^-1 showed no significant difference. The results suggest that it may be possible for FTIR to become an effective method to estimate the radiation dose in clinic.