@inproceedings{b26227a0dcb249ccaa44088c6bbc14c5,
title = "Study of non-viral gene transfer of NB4 cells",
abstract = "Post-Transcriptional Gene Silencing and RNA interference (RNAi) are terms describing the specific suppression of genes by complementary dsRNA. High-efficiency transfection is an essential first step for achieving effective gene knockdown. Non-viral transfection include chemical-transfection and electrotransfection. For a particular cell line, select the appropriate transfection method is very important. In this study, we compared a variety of commercially available reagents and electrotransfection, approaches to define methods for efficient delivery of siRNA to NB4 cells. Fluorescently-labelled siRNA were tested. We report that different methods have different effects. The results provide guidelines for optimal siRNA-mediated knockdown in the NB4 cells.",
keywords = "NB4 cells, RNAi, Transfection",
author = "Ying Chen and Huaiyu Wang and Mei Zhang",
year = "2012",
doi = "10.4028/www.scientific.net/AMR.341-342.333",
language = "英语",
isbn = "9783037852521",
series = "Advanced Materials Research",
pages = "333--337",
booktitle = "Material and Manufacturing Technology II",
note = "2011 2nd International Conference on Material and Manufacturing Technology, ICMMT 2011 ; Conference date: 08-07-2011 Through 11-07-2011",
}