Studies on the kinetic properties of photosystem II primary reaction using fluorescence spectroscopy with 470 fs time resolution

The primary reaction kinetics of the isolated photosystem II particles and photosystem II core complexes from spinach (Spinacia deracea Mill.) was investigated using the time-resolved fluo-rescence spectroscopy with 470 fs time resolution. 2 to 4 lifetime components were detected by the multi-exponential curve fitting method. These components were analyzed and discussed in terms of different kinetic processes. It is suggested that 3 ps component is attributed to the charge separation and 0.8 ps, 12 ps, 25 ps and 100 ps components are related to the energy transfer processes. A possible kinetic scheme in photosystem II reaction center was proposed based upon the reported previously result.