Structural Basis of Phosphatidic Acid Sensing by APH in Apicomplexan Parasites

Plasmodium falciparum and Toxoplasma gondii are obligate intracellular parasites that belong to the phylum of Apicomplexa and cause major human diseases. Their access to an intracellular lifestyle is reliant on the coordinated release of proteins from the specialized apical organelles called micronemes and rhoptries. A specific phosphatidic acid effector, the acylated pleckstrin homology domain-containing protein (APH) plays a central role in microneme exocytosis and thus is essential for motility, cell entry, and egress. TgAPH is acylated on the surface of the micronemes and recruited to phosphatidic acid (PA)-enriched membranes. Here, we dissect the atomic details of APH PA-sensing hub and its functional interaction with phospholipid membranes. We unravel the key determinant of PA recognition for the first time and show that APH inserts into and clusters multiple phosphate head-groups at the bilayer binding surface. High-resolution structures of a phosphatidic acid effector protein (APH) from both Plasmodium falciparum and Toxoplasma gondii parasites and its interaction with the lipid mediator unravel a new class of PH domain that initiates microneme-plasma membrane fusion by inserting and clustering phosphatidic acid within membranes.