Single and joint effects of acetochlor and urea on earthworm Esisenia foelide populations in phaiozem

Much attention is paid to soil health and environmental safety. Earthworms are an important indicator of soil ecosystem health and safety. Ecological toxicity of acetochlor and excessive urea, in both their single and joint effects, on earthworm Esisenia foelide was thus studied using the soil-culture method. Acetochlor had an enhanced toxicity from low concentration to high concentration. The mortality of earthworms after a 6-day exposure was changed from 0 to 86.7%, and the weight change rate ranged from 7.86 to - 30.43%, when the concentration of acetochlor was increased from 164 to 730 mg kg^-1. Urea expressed its positive and beneficial effects on earthworms when its concentration was lower than 500 mg kg^-1. Strongly toxic effects took place when the concentration of urea was higher than 1000 mg kg^-1. The mortality of earthworms exposed to urea reached 100% when its concentration was more than 1500 mg kg^-1. When the concentration of urea was lower than 500 mg kg^-1, there were antagonistic effects between the two agrochemicals on earthworms; when the concentration of urea was higher than 500 mg kg^-1, joint toxic effects of acetochlor and excessive urea on earthworms were synergic. In any case, excessive urea application is very harmful to the health of soil ecosystems.