TY - JOUR
T1 - Separation and purification of human salivary protein by high-performance hydrophobicity chromatography
AU - Jing, Juan
AU - Hou, Tiezhou
AU - Zhao, Dongfang
AU - Wang, Qiang
AU - Ruan, Meisheng
AU - Hou, Jianxia
PY - 2003/2
Y1 - 2003/2
N2 - Objective: To separate and purify human whole salivary protein, parotid salivary protein and submandibular/sublingual salivary protein by HPHIC to discuss the best separation condition and its clinical significance. Methods: Each kind of salivary sample was collected, centrifugated and lyophilized at low temperature and stored for use. After the gradient was separated by different concentrations of (NH4)2SO4, KH2 PO4 as A and B phase, each spike was collected and qualified by biochemical techniques. Results: There were 12, 6 and 8 protein components in the whole salivary, parotid salivary and submandibular/sublingual salivary, respectively, with minimal standard error and good duplication. Five kinds of protein could be qualified, such as proline-rich protein, statherin, cystatin S, α-amylase and histone-rich protein. Conclusion: HPHIC can be used to separate human whole salivary protein, parotid salivary potein and submandibular/sublingual salivary protein.
AB - Objective: To separate and purify human whole salivary protein, parotid salivary protein and submandibular/sublingual salivary protein by HPHIC to discuss the best separation condition and its clinical significance. Methods: Each kind of salivary sample was collected, centrifugated and lyophilized at low temperature and stored for use. After the gradient was separated by different concentrations of (NH4)2SO4, KH2 PO4 as A and B phase, each spike was collected and qualified by biochemical techniques. Results: There were 12, 6 and 8 protein components in the whole salivary, parotid salivary and submandibular/sublingual salivary, respectively, with minimal standard error and good duplication. Five kinds of protein could be qualified, such as proline-rich protein, statherin, cystatin S, α-amylase and histone-rich protein. Conclusion: HPHIC can be used to separate human whole salivary protein, parotid salivary potein and submandibular/sublingual salivary protein.
KW - Chromatography (HPHIC)
KW - High-performance hydrophobicity
KW - Parotid salivary
KW - Purification
KW - Salivary protein
KW - Separation
KW - Submandibular/sublingual salivary
KW - Whole salivary
UR - https://www.scopus.com/pages/publications/0038268871
M3 - 文章
AN - SCOPUS:0038268871
SN - 1671-8259
VL - 24
SP - 82
EP - 84
JO - Journal of Xi'an Jiaotong University (Medical Sciences)
JF - Journal of Xi'an Jiaotong University (Medical Sciences)
IS - 1
ER -