Screening of gene encoding of hepatic proteins interacting with Hcbp6 via yeast two hybridization

AIM: To seek for hepatic proteins that interacted with protein encoded by Hcbp6 for exploring the biological function of Hcbp6. METHODS: Hcbp6 gene was introduced into pGBKT7, and then transformed into yeast AH109, which was mated with yeast Y187 (α type) containing liver cDNA library plasmid in 2xYPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing x-α-gal. Plasmids were extracted from positive colonies, and sequence analysis was performed by bioinformatics. RESULTS: Four kind of proteins including paralemmin, Ran binding protein 2, transmembrane transporting protein and albumin were identified to interact with Hcbp6 specifically. CONCLUSION: Hcbp6 proteins may belong to or be associated with formation of secretary proteins, more study needs to be done for clarifying its biological function.