TY - JOUR
T1 - Role of circular RNAs in formaldehyde-induced lung Injury
T2 - Insights into expression and regulatory pathways
AU - Ge, Pan
AU - Zhang, Xiang
AU - Yang, Yan qi
AU - Xie, Jia ming
AU - Lv, Mo qi
AU - Sun, Ying
AU - Li, Ya zhao
AU - Geng, Yuan yuan
AU - Zhang, Jian
AU - Zhou, Dang xia
N1 - Publisher Copyright:
© 2025
PY - 2025/9
Y1 - 2025/9
N2 - Background: Accumulating evidence indicates that formaldehyde influence the pathogenesis of lung injury; however, the molecular mechanisms are unclear. This study aimed to investigate the expression profiles and function of circular RNAs (circRNAs) in formaldehyde-induced lung injury. Methods: Differentially expressed circRNA profiles in formaldehyde-exposed rat lung tissue were identified using circRNA microarray. Subsequently, circRNA expression levels were validated. The potential circRNA/miRNA/mRNA regulatory networks were predicted and confirmed through bioinformatics analysis, dual-luciferase reporter assays, and siRNA transfection experiments. Results: A total of 3932 differentially expressed circRNAs were identified in formaldehyde-exposed lung tissues, comprising 2604 upregulated and 1328 downregulated circRNAs. Notably, rno_circRNA_010133 (increased 225 %, P < 0.01), rno_circRNA_016194 (increased 189.42 %, P < 0.01), and rno_circRNA_008646 (increased 93.2 %, P < 0.05) were significantly upregulated, whereas rno_circRNA_002357 (decreased 47.57 %, P < 0.01), rno_circRNA_004857 (decreased 81.55 %, P < 0.01), and rno_circRNA_002815 (decreased 66.99 %, P < 0.01) were markedly downregulated in formaldehyde-exposed lung tissues, consistent with microarray data. Additionally, the rno_circRNA_010133/rno-miR-107/Atg12 axis was activated, while the rno_circRNA_002357/rno-miR-9a-5p/Wnt pathway was suppressed in formaldehyde-exposed lung tissues. Conclusions: This study provided novel insights into the altered expression patterns of circRNAs in formaldehyde-induced lung injury in rats, offering valuable information for the prevention and treatment of occupational formaldehyde-related lung injuries.
AB - Background: Accumulating evidence indicates that formaldehyde influence the pathogenesis of lung injury; however, the molecular mechanisms are unclear. This study aimed to investigate the expression profiles and function of circular RNAs (circRNAs) in formaldehyde-induced lung injury. Methods: Differentially expressed circRNA profiles in formaldehyde-exposed rat lung tissue were identified using circRNA microarray. Subsequently, circRNA expression levels were validated. The potential circRNA/miRNA/mRNA regulatory networks were predicted and confirmed through bioinformatics analysis, dual-luciferase reporter assays, and siRNA transfection experiments. Results: A total of 3932 differentially expressed circRNAs were identified in formaldehyde-exposed lung tissues, comprising 2604 upregulated and 1328 downregulated circRNAs. Notably, rno_circRNA_010133 (increased 225 %, P < 0.01), rno_circRNA_016194 (increased 189.42 %, P < 0.01), and rno_circRNA_008646 (increased 93.2 %, P < 0.05) were significantly upregulated, whereas rno_circRNA_002357 (decreased 47.57 %, P < 0.01), rno_circRNA_004857 (decreased 81.55 %, P < 0.01), and rno_circRNA_002815 (decreased 66.99 %, P < 0.01) were markedly downregulated in formaldehyde-exposed lung tissues, consistent with microarray data. Additionally, the rno_circRNA_010133/rno-miR-107/Atg12 axis was activated, while the rno_circRNA_002357/rno-miR-9a-5p/Wnt pathway was suppressed in formaldehyde-exposed lung tissues. Conclusions: This study provided novel insights into the altered expression patterns of circRNAs in formaldehyde-induced lung injury in rats, offering valuable information for the prevention and treatment of occupational formaldehyde-related lung injuries.
KW - CircRNA microarray
KW - Inhalation exposure
KW - Regulatory pathways
KW - Toxicology
UR - https://www.scopus.com/pages/publications/105009221332
U2 - 10.1016/j.fct.2025.115604
DO - 10.1016/j.fct.2025.115604
M3 - 文章
C2 - 40578478
AN - SCOPUS:105009221332
SN - 0278-6915
VL - 203
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
M1 - 115604
ER -