TY - JOUR
T1 - Review on isotachophoresis in trace biochemical sample analysis
T2 - state of art and future prospects
AU - Mao, Qi
AU - Zhu, Wangang
AU - Luo, Adam Ganxi
AU - Yao, Honghong
AU - Han, Bing
AU - Gancheva, Veska
AU - Correia, Jose Higino
AU - Zhao, Hong
AU - Lin, Zhiming
AU - Li, Zhecong
AU - Hao, Bohua
AU - Lu, Junyu
AU - Huang, Guangming
AU - Xu, Tao
AU - Huang, Liang
AU - Hu, Kui
AU - Zhu, Minjie
AU - Ouyang, Jingsong
AU - Ferre, Xavier Mateos
AU - Karlsen, Haakon
AU - Sampietro, Marco
AU - Wang, Zhuqing
AU - Dong, Tao
N1 - Publisher Copyright:
© 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
PY - 2026/9/27
Y1 - 2026/9/27
N2 - Isotachophoresis (ITP) is a green, efficient, and convenient technology for online sample purification and enrichment. Sample concentration is accomplished by leveraging differences in ion mobility within an electric field between the leading electrolyte and the trailing electrolyte. Over time, ITP devices have shown substantial improvements in sensitivity and detection limits for nucleic acid analysis. Additionally, the range of biological samples tested and disease-related applications has expanded considerably. This review principally emphasizes application progress of ITP in trace biochemical sample analysis especially nucleic acids. In particular, it provides an overview of common coupling techniques. Furthermore, the key parameters for ITP implementation in trace biochemical sample purification and enrichment are comprehensively summarized.
AB - Isotachophoresis (ITP) is a green, efficient, and convenient technology for online sample purification and enrichment. Sample concentration is accomplished by leveraging differences in ion mobility within an electric field between the leading electrolyte and the trailing electrolyte. Over time, ITP devices have shown substantial improvements in sensitivity and detection limits for nucleic acid analysis. Additionally, the range of biological samples tested and disease-related applications has expanded considerably. This review principally emphasizes application progress of ITP in trace biochemical sample analysis especially nucleic acids. In particular, it provides an overview of common coupling techniques. Furthermore, the key parameters for ITP implementation in trace biochemical sample purification and enrichment are comprehensively summarized.
KW - Enrichment
KW - Isotachophoresis
KW - Purification
KW - Trace biochemical sample
UR - https://www.scopus.com/pages/publications/105044590521
U2 - 10.1016/j.chroma.2026.467215
DO - 10.1016/j.chroma.2026.467215
M3 - 文献综述
AN - SCOPUS:105044590521
SN - 0021-9673
VL - 1785
JO - Journal of Chromatography A
JF - Journal of Chromatography A
M1 - 467215
ER -