Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide

Bing Liu; James A. Garnett; Wei chao Lee; Jing Lin; Paula Salgado; Jonathan Taylor; Yingqi Xu; Sebastian Lambert; Ernesto Cota; Steve Matthews

doi:10.1016/j.bbrc.2012.03.136

Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide

Bing Liu
, James A. Garnett
, Wei chao Lee
, Jing Lin
, Paula Salgado
, Jonathan Taylor
, Yingqi Xu
, Sebastian Lambert
, Ernesto Cota
, Steve Matthews

Imperial College London

科研成果: 期刊稿件 › 文章 › 同行评审

5 引用（Scopus）

摘要

The use of heavy water (D ₂O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D ₂O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.

源语言	英语
页（从-至）	208-213
页数	6
期刊	Biochemical and Biophysical Research Communications
卷	421
期	2
DOI	https://doi.org/10.1016/j.bbrc.2012.03.136
出版状态	已出版 - 4 5月 2012
已对外发布	是

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title = "Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide",

abstract = "The use of heavy water (D 2O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D 2O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.",

keywords = "Additive, Crystallography, Deuterium oxide, Pilin",

author = "Bing Liu and Garnett, \{James A.\} and Lee, \{Wei chao\} and Jing Lin and Paula Salgado and Jonathan Taylor and Yingqi Xu and Sebastian Lambert and Ernesto Cota and Steve Matthews",

year = "2012",

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doi = "10.1016/j.bbrc.2012.03.136",

language = "英语",

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T1 - Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide

AU - Liu, Bing

AU - Garnett, James A.

AU - Lee, Wei chao

AU - Lin, Jing

AU - Salgado, Paula

AU - Taylor, Jonathan

AU - Xu, Yingqi

AU - Lambert, Sebastian

AU - Cota, Ernesto

AU - Matthews, Steve

PY - 2012/5/4

Y1 - 2012/5/4

N2 - The use of heavy water (D 2O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D 2O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.

AB - The use of heavy water (D 2O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D 2O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.

KW - Additive

KW - Crystallography

KW - Deuterium oxide

KW - Pilin

UR - https://www.scopus.com/pages/publications/84860450242

U2 - 10.1016/j.bbrc.2012.03.136

DO - 10.1016/j.bbrc.2012.03.136

M3 - 文章

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AN - SCOPUS:84860450242

SN - 0006-291X

VL - 421

SP - 208

EP - 213

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide

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