Observation of the immunity of normal rats injected with leukemia vaccine

Objective: To observe the general status and the immunity of C ₅₇BL/6 rats injected with a kind of leukemia vaccine and to observe the survival rate of these injected rats that were then attacked by wild FBL-3 cells. Methods: C₅₇ BL/6 rats were injected subcutaneuosly with a kind of leukemia vaccine, which was made from inactived FBL-3 cells additional of IFA and cytokines, such as rGM-CSF, rIL-2 and rIL-6. Then these rats were attacked by subcutaneous injection of wild FBL-3 cells after 2 weeks. The general status, the rate of tumor formation and the growth of tumor were recorded; the site of the vaccine injected and the site of FBL-3 injected were observed with pathological method; the cytotoxicity of Mφ and CTL derived from these rats were tested by the MTT colorimetric method. Results: Compared with controls, this vaccine could promote the cellar immunity of C ₅₇BL/6 rats. The rate of tumor formation was lower, the cytotoxicity of Mφ and CTL was higher, the growth of tumor was slower, the survival time was longer in the test group than that in the control group. The necrosis in the tumor node was much more obvious, the infiltration of mononuclear cells both in the tumor node and in the site of vaccine injection was much more obvious in the test group than that in the control group. Conclusion: Prepared in a simple way, the leukemia vaccine has definite effects and few side effects. However, further research is needed.