TY - JOUR
T1 - O-linked N-acetylglucosamine transferase (OGT) is overexpressed and promotes O-linked protein glycosylation in esophageal squamous cell carcinoma
AU - Qiao, Zhe
AU - Dang, Chengxue
AU - Zhou, Bin
AU - Li, Shaomin
AU - Zhang, Wei
AU - Jiang, Jiantao
AU - Zhang, Jin
AU - Kong, Ranran
AU - Ma, Yuefeng
PY - 2012/7
Y1 - 2012/7
N2 - The aim of this present study was to explore the expression and clinical significance of O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) and enzymatic O-linked glycosylation (O-GlcNAcation) through the addition of O-linked-βN-acetylglucosamine in esophageal squamous cell carcinoma. OGT expression and O-GlcNAcation in 40 samples from patients with esophageal squamous cell carcinoma was detected by im-munohistochemical staining with anti-OGT antib ody and O-GlcNAc-specific antibody RL 2, respectively. The relationship between pathological and clinical factors of patients was analyzed. We found that the expression of OGT was higher in esophageal squamous cell carcinoma samples compared to the normal tissues. RL 2 antibody level was positively correlated with OGT expression, and the metastasis of lymph node, which means the level of O-GlcNAcation was high and related to the metastasis of lymph node in esophageal squamous cell carcinoma. In conclusion, OGT activation is the main reason for promoting the level of O-GlcNAcation in esophageal squamous cell carcinoma. O-GlcNAcylation may play an important role in esophageal squamous cell carcinoma.
AB - The aim of this present study was to explore the expression and clinical significance of O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) and enzymatic O-linked glycosylation (O-GlcNAcation) through the addition of O-linked-βN-acetylglucosamine in esophageal squamous cell carcinoma. OGT expression and O-GlcNAcation in 40 samples from patients with esophageal squamous cell carcinoma was detected by im-munohistochemical staining with anti-OGT antib ody and O-GlcNAc-specific antibody RL 2, respectively. The relationship between pathological and clinical factors of patients was analyzed. We found that the expression of OGT was higher in esophageal squamous cell carcinoma samples compared to the normal tissues. RL 2 antibody level was positively correlated with OGT expression, and the metastasis of lymph node, which means the level of O-GlcNAcation was high and related to the metastasis of lymph node in esophageal squamous cell carcinoma. In conclusion, OGT activation is the main reason for promoting the level of O-GlcNAcation in esophageal squamous cell carcinoma. O-GlcNAcylation may play an important role in esophageal squamous cell carcinoma.
KW - Esophageal squamous cell carcinoma
KW - O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT)
KW - O-linked glycosylation (O-GlcNA-cation)
UR - https://www.scopus.com/pages/publications/84864758444
U2 - 10.7555/JBR.26.20110121
DO - 10.7555/JBR.26.20110121
M3 - 文章
AN - SCOPUS:84864758444
SN - 1674-8301
VL - 26
SP - 268
EP - 273
JO - Journal of Biomedical Research
JF - Journal of Biomedical Research
IS - 4
ER -