Novel solid-phase extraction filter based on a zirconium meta-organic framework for determination of non-steroidal anti-inflammatory drugs residues

In this study, a zirconium-based metal-organic framework UiO-66-NH₂ modified cotton fiber (CF@UiO-66-NH₂) was fabricated for the extraction of five common NSAIDs, namely ketoprofen, naproxen, flurbiprofen, diclofenac sodium, and ibuprofen. UiO-66-NH₂ was synthesized and immobilized on the surface of cotton fiber using an environmentally friendly aqueous synthesis method. The prepared CF@UiO-66-NH₂ composite of 50 mg was loaded into a 13 mm recessed filter for use as a solid-phase extraction (SPE) adsorbent material. The filter was then used to enrich NSAIDs in fish and shrimp muscle tissues followed by ultra-high performance liquid chromatography (UPLC) detection. Several key parameters were evaluated and optimized, including adsorption flow rate, pH value of sample, desorption flow rate, and the formic acid content of the eluent. Under optimized conditions, linear ranges of ketoprofen, naproxen, flurbiprofen, diclofenac sodium, and ibuprofen were 2.0–300.0 ng/mL, 1.4–280.0 ng/mL, 3.0–400.0 ng/mL, 1.0–500.0 ng/mL, and 14.0–560.0 ng/mL, respectively. The detection limits ranged from 0.12 ng/mL to 3.50 ng/mL with recoveries in the range of 72.95–116.99%, RSDs < 9.90%. The results demonstrated that the homemade filters based on CF@UiO-66-NH₂ exhibited good reproducibility, stability and adsorption property for the determination of trace-level NSAIDs in complex matrix.