TY - JOUR
T1 - New method of screening allergenic components from Shuanghuanglian injection
T2 - With RBL-2H3/CMC model online HPLC/MS system
AU - Han, Shengli
AU - Zhang, Tao
AU - Huang, Jing
AU - Cui, Ronghua
AU - He, Langchong
PY - 2014/1/25
Y1 - 2014/1/25
N2 - Shuanghuanglian (SHL) injection is a traditional Chinese medicine (TCM) injection widely used in China to treat influenza, tonsillitis, bronchitis, and faucitis. Adverse drug reactions (ADR) of SHL injection, majorly manifested as allergic reactions, were among the leading causes of death from TCM injection. In this study, an RBL-2H3/CMC online LC/MS system was established to screen and identify allergenic components in SHL injection, by which Baicalin was identified as a potential allergenic component. Allergenic activities of baicalin were investigated in RBL-2H3 cell degranulation and β-hexosaminidase release tests in vitro. Our results showed that baicalin dose-dependently induced RBL-2H3 cell degranulation and β-hexosaminidase release in the range of 0-50. μg/ml. The RBL-2H3/CMC online LC/MS system developed in this study may potentially be used to screen allergenic components in other TCM injections.
AB - Shuanghuanglian (SHL) injection is a traditional Chinese medicine (TCM) injection widely used in China to treat influenza, tonsillitis, bronchitis, and faucitis. Adverse drug reactions (ADR) of SHL injection, majorly manifested as allergic reactions, were among the leading causes of death from TCM injection. In this study, an RBL-2H3/CMC online LC/MS system was established to screen and identify allergenic components in SHL injection, by which Baicalin was identified as a potential allergenic component. Allergenic activities of baicalin were investigated in RBL-2H3 cell degranulation and β-hexosaminidase release tests in vitro. Our results showed that baicalin dose-dependently induced RBL-2H3 cell degranulation and β-hexosaminidase release in the range of 0-50. μg/ml. The RBL-2H3/CMC online LC/MS system developed in this study may potentially be used to screen allergenic components in other TCM injections.
KW - Anaphylactoid reaction
KW - Cell degranulation
KW - Liquid chromatography tandem mass spectrum
KW - RBL-2H3 cell membrane chromatography
KW - β-Hexosaminidase release
UR - https://www.scopus.com/pages/publications/84887369421
U2 - 10.1016/j.jpba.2013.10.006
DO - 10.1016/j.jpba.2013.10.006
M3 - 文章
C2 - 24211723
AN - SCOPUS:84887369421
SN - 0731-7085
VL - 88
SP - 602
EP - 608
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -