Modification, characterization and structure-anticoagulant activity relationships of persimmon polysaccharides

Six chemical sulfated polysaccharide derivatives (PFP-S) with variable degree of substitution (DS) and molecular weights (Mw) were prepared from the fresh persimmon fruits by chlorosulfonic acid-pyridine and ultrasonic degradation methods, and the effectiveness of the reaction was monitored by the DS, FT-IR and ζ-potential analysis. The sulfation modification might cause advanced structure change to the samples, the SEM of PFP-S exhibited a polyphasic convoluted structure. Results of anticoagulant activities showed that PFP-S could prolong activated partial thromboplastin time (APTT) and thrombin time (TT), but not prothrombin time (PT), and the anticoagulant activity improved with the increasing DS and decreasing Mw.