Mixed-model of affinity and hydrophobic interaction for drug retention in cell membrane chromatography

According to the stoichiometric displacement concept and some reasonable assumptions for the surface property and the interaction between the cell membrane stationary phase (CMSP) and drugs, a mixed model of affinity and hydrophobic interaction for drug retention on the CMSP is reported. An equation expressing the "mixed mechanism" was tested by five kinds of calcium antagonists and a rabbit myocardium CMSP with three kinds of different mobile phases. The experimental results basically coincide with that of the theoretical expectation.