miRNA-449 family inhibits gastric cancer cells by targeting CCNE2

Di Liu; Chen Huang; Dong Mei Diao; Jian Guang Wang; Yao Cheng; Lei Peng Ren; Cheng Xue Dang

miRNA-449 family inhibits gastric cancer cells by targeting CCNE2

Di Liu
, Chen Huang
, Dong Mei Diao
, Jian Guang Wang
, Yao Cheng
, Lei Peng Ren
, Cheng Xue Dang

医学部

Xi'an Jiaotong University

科研成果: 期刊稿件 › 文章 › 同行评审

摘要

Objective: To study the effects of miRNA-449a/b on apoptosis of gastric cancer cells and expression of its target CCNE2. Methods: The expression of miRNA-449a/b in gastric cancer cell line BGC823 after transient transfection was detected by Real-time PCR. Flow cytometry was used to detect cell apoptosis rate. The target gene of miR-449a/b was detected by Western blot. Results: At 48 and 72 h, miRNA-449a/b was overexpressed in transient transfection cells and the upregulated miRNA-449a/b could inhibit proliferation and promote apoptosis (P<0.05). The expression of CCNE2 was also inhibited significantly (P<0.05). Conclusion: miRNA-449a/b inhibits gastric cancer cell probably by targeting CCNE2.

源语言	英语
页（从-至）	544-548
页数	5
期刊	Journal of Xi'an Jiaotong University (Medical Sciences)
卷	33
期	5
出版状态	已出版 - 9月 2012

联合国可持续发展目标

此成果有助于实现下列可持续发展目标：

可持续发展目标 3 良好健康与福祉

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链接到 Scopus 的出版物

引用此

@article{d42f2eaf9b7d477eab1ac29a05cdfefd,

title = "miRNA-449 family inhibits gastric cancer cells by targeting CCNE2",

abstract = "Objective: To study the effects of miRNA-449a/b on apoptosis of gastric cancer cells and expression of its target CCNE2. Methods: The expression of miRNA-449a/b in gastric cancer cell line BGC823 after transient transfection was detected by Real-time PCR. Flow cytometry was used to detect cell apoptosis rate. The target gene of miR-449a/b was detected by Western blot. Results: At 48 and 72 h, miRNA-449a/b was overexpressed in transient transfection cells and the upregulated miRNA-449a/b could inhibit proliferation and promote apoptosis (P<0.05). The expression of CCNE2 was also inhibited significantly (P<0.05). Conclusion: miRNA-449a/b inhibits gastric cancer cell probably by targeting CCNE2.",

keywords = "Apoptosis, CCNE2 (cyclin E2), Cell cycle, Gastric cancer cell, miRNA",

author = "Di Liu and Chen Huang and Diao, \{Dong Mei\} and Wang, \{Jian Guang\} and Yao Cheng and Ren, \{Lei Peng\} and Dang, \{Cheng Xue\}",

year = "2012",

month = sep,

language = "英语",

volume = "33",

pages = "544--548",

journal = "Journal of Xi'an Jiaotong University (Medical Sciences)",

issn = "1671-8259",

publisher = "Xi'an Medical University",

number = "5",

}

TY - JOUR

T1 - miRNA-449 family inhibits gastric cancer cells by targeting CCNE2

AU - Liu, Di

AU - Huang, Chen

AU - Diao, Dong Mei

AU - Wang, Jian Guang

AU - Cheng, Yao

AU - Ren, Lei Peng

AU - Dang, Cheng Xue

PY - 2012/9

Y1 - 2012/9

N2 - Objective: To study the effects of miRNA-449a/b on apoptosis of gastric cancer cells and expression of its target CCNE2. Methods: The expression of miRNA-449a/b in gastric cancer cell line BGC823 after transient transfection was detected by Real-time PCR. Flow cytometry was used to detect cell apoptosis rate. The target gene of miR-449a/b was detected by Western blot. Results: At 48 and 72 h, miRNA-449a/b was overexpressed in transient transfection cells and the upregulated miRNA-449a/b could inhibit proliferation and promote apoptosis (P<0.05). The expression of CCNE2 was also inhibited significantly (P<0.05). Conclusion: miRNA-449a/b inhibits gastric cancer cell probably by targeting CCNE2.

AB - Objective: To study the effects of miRNA-449a/b on apoptosis of gastric cancer cells and expression of its target CCNE2. Methods: The expression of miRNA-449a/b in gastric cancer cell line BGC823 after transient transfection was detected by Real-time PCR. Flow cytometry was used to detect cell apoptosis rate. The target gene of miR-449a/b was detected by Western blot. Results: At 48 and 72 h, miRNA-449a/b was overexpressed in transient transfection cells and the upregulated miRNA-449a/b could inhibit proliferation and promote apoptosis (P<0.05). The expression of CCNE2 was also inhibited significantly (P<0.05). Conclusion: miRNA-449a/b inhibits gastric cancer cell probably by targeting CCNE2.

KW - Apoptosis

KW - CCNE2 (cyclin E2)

KW - Cell cycle

KW - Gastric cancer cell

KW - miRNA

UR - https://www.scopus.com/pages/publications/84866158348

M3 - 文章

AN - SCOPUS:84866158348

SN - 1671-8259

VL - 33

SP - 544

EP - 548

JO - Journal of Xi'an Jiaotong University (Medical Sciences)

JF - Journal of Xi'an Jiaotong University (Medical Sciences)

IS - 5

ER -

miRNA-449 family inhibits gastric cancer cells by targeting CCNE2

摘要

联合国可持续发展目标

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