Magnetic covalent organic framework immobilized gold nanoparticles with high-efficiency catalytic performance for chemiluminescent detection of pesticide triazophos

Covalent organic frameworks (COFs) are considered to be a promising support material for catalyst due to their highly ordered porous structure. Here, a core-shell structured Fe₃O₄ magnetic covalent organic framework (Fe₃O₄@COF) was synthesized and employed to provide basic sites for immobilization of gold nanoparticles (AuNPs). The AuNPs was in-situ immobilized on the shell of Fe₃O₄@COF via a citrate reducing method. The Fe₃O₄@COF-AuNP had convenient magnetic separability and exhibited excellent mimicking peroxidase-like activity in catalyzing chemiluminescence (CL) reaction of luminol with hydrogen peroxide (H₂O₂). With acetylcholine chloride (ACh) as substrate of acetylcholinesterase (AChE), a CL method was exploited for sensitive detection of organophosphorus pesticide triazophos due to its irreversible inhibiting effect on the AChE activity and subsequently influences the production of H₂O₂ under the condition of choline oxidase (ChOx). This method gave a good linearity for triazophos in the range of 5.0–300.0 nmol L⁻¹, and a limit of detection (LOD) of 1 nmol L⁻¹ was acquired. The applicability of this method was verified by the determination of triazophos in different spiked vegetable samples.