TY - JOUR
T1 - Involvement of ventrolateral orbital cortex 5-HT 1-7 receptors in 5-HT induced depression of spared nerve injury allodynia
AU - Xu, W. J.
AU - Zhao, Y.
AU - Huo, F. Q.
AU - Du, J. Q.
AU - Tang, J. S.
PY - 2013/5/5
Y1 - 2013/5/5
N2 - The present study examined the roles of ventrolateral orbital cortex (VLO) 5-HT1, 5-HT2, 5-HT3, 5-HT4, 5-HT5, 5-HT6 and 5-HT7 receptor subtypes in mediating 5-HT-induced antiallodynic actions in the rat spared nerve injury (SNI) pain model. Changes in paw withdrawal threshold (PWT) were measured using von-Frey filaments. Microinjection of 5-HT (2, 5 and 10μg, in 0.5μl) into the VLO depressed allodynia induced by SNI, and the PWT increased in a dose-dependent manner. Microinjection of selective 5-HT1A, 5-HT2, 5-HT3, 5-HT4, 5-HT5A, 5-HT6 and 5-HT7 receptor antagonists, 1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl] piperazine hydrobromide (NAN-190) (10μg), cyproheptadine (50ng), granisetron hydrochloride (granisetron) (10μg), 1-[2-[(methylsulfonyl)-amino]ethyl]-4-piperidinyl]methyl1-methyl-1H-indole-3-carboxylate (GR113808) (5μg), SB699551 dihydrochloride (SB699551) (10μg), SB258585 dihydrochloride (SB258585) (2μg) or SB269970 hydrochloride (SB269970) (10μg) into the VLO 5-min prior to 5-HT (10μg) injection, all antagonized the 5-HT-induced inhibition of allodynia. In addition, these antagonists applied alone to VLO did not influence allodynia. These results suggest that although 5-HT1-7 receptor subtypes in the VLO do not have a tonic modulatory action on the allodynia induced by SNI, they are involved in mediating the depression of the SNI allodynia produced by injection of 5HT into VLO.
AB - The present study examined the roles of ventrolateral orbital cortex (VLO) 5-HT1, 5-HT2, 5-HT3, 5-HT4, 5-HT5, 5-HT6 and 5-HT7 receptor subtypes in mediating 5-HT-induced antiallodynic actions in the rat spared nerve injury (SNI) pain model. Changes in paw withdrawal threshold (PWT) were measured using von-Frey filaments. Microinjection of 5-HT (2, 5 and 10μg, in 0.5μl) into the VLO depressed allodynia induced by SNI, and the PWT increased in a dose-dependent manner. Microinjection of selective 5-HT1A, 5-HT2, 5-HT3, 5-HT4, 5-HT5A, 5-HT6 and 5-HT7 receptor antagonists, 1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl] piperazine hydrobromide (NAN-190) (10μg), cyproheptadine (50ng), granisetron hydrochloride (granisetron) (10μg), 1-[2-[(methylsulfonyl)-amino]ethyl]-4-piperidinyl]methyl1-methyl-1H-indole-3-carboxylate (GR113808) (5μg), SB699551 dihydrochloride (SB699551) (10μg), SB258585 dihydrochloride (SB258585) (2μg) or SB269970 hydrochloride (SB269970) (10μg) into the VLO 5-min prior to 5-HT (10μg) injection, all antagonized the 5-HT-induced inhibition of allodynia. In addition, these antagonists applied alone to VLO did not influence allodynia. These results suggest that although 5-HT1-7 receptor subtypes in the VLO do not have a tonic modulatory action on the allodynia induced by SNI, they are involved in mediating the depression of the SNI allodynia produced by injection of 5HT into VLO.
KW - 5-HT receptor subtypes
KW - 5-hydroxytryptamine
KW - Allodynia
KW - Neuropathic pain
KW - Pain modulation.
KW - Ventrolateral orbital cortex
UR - https://www.scopus.com/pages/publications/84875843754
U2 - 10.1016/j.neuroscience.2013.02.036
DO - 10.1016/j.neuroscience.2013.02.036
M3 - 文章
C2 - 23485814
AN - SCOPUS:84875843754
SN - 0306-4522
VL - 238
SP - 252
EP - 257
JO - Neuroscience
JF - Neuroscience
ER -