Investigations on colorectum cancer cell lines and nucleus by FTIR spectroscopy

The purpose of this study is to establish the cell and subcellular basis for the diagnosis of colorectal cancer by researching the FTIR (Fourier transform infrared spectra) of the normal tissue (30 cases) and cell line (SW 620), normal nuclei and malignant nucleus with Fourier transform infrared spectrum technology. The results of the peak position and the relative intensity obtained by statistical analysis indicate that the bands at 2925, 1240 and 1085 cm^-1 shifted toward higher wavenumber (p<0.05) in the FTIR spectra of cell lines (SW 620) and malignant nucleus. The 1400 cm^-1 band shifted toward lower wave number (p<0.05). The relative intensity ratios of I₁₆₅₀/I₁₄₆₀, I₁₄₀₀/I₁₄₆₀ and I₁₂₄₀/I₁₄₆₀ increased in the FTIR spectra of cell lines (SW 620) compared with normal tissue (p<0.05), but inversely, I₁₇₄₀/I₁₄₆₀ decreased significantly (p<0.01). The relative intensity ratios of I₁₆₅₀/I₁₄₆₀, I₁₄₀₀/I₁₄₆₀ and I₁₂₄₀/I₁₄₆₀ also increased in the FTIR spectra of the malignant nucleus. The above statistic differences are the basis for diagnosing colorectum cancer by FTIR spectroscopy.