Inhibitory action of docetaxel on the proliferation of HeLa and SiHa cells

Objective: To study the inhibitory action of docetaxel (DOC) on the proliferation of HeLa and SiHa cells. Methods: Cell morphological changes were observed with inverted phase contrast microscope. MTT was adopted to test and calculate the cell inhibition ratio. Flow cytometry was used to detect cell cycle. Results: DOC had an obvious concentration-dependent inhibitory effect on the proliferation of both HeLa and SiHa cells. The inhibition ratio of DOC on SiHa was significantly higher than that on HeLa (P<0.05). DOC blocked HeLa at G₂/M phase. Under the effect of DOC, the cell cycle of SiHa was not changed much. Conclusion: DOC has an obvious inhibitory action on both HeLa and SiHa cells, which shows a promising prospect of DOC in clinical treatment of cervical cancer.