In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions

Sheng Li; Guoan Zhang; Yanping Liu; Qinru Sun; Lu Yang; Junli Dong; Bowen Zhang; Su Chen

doi:10.1016/j.xpro.2021.100601

In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions

Sheng Li
, Guoan Zhang
, Yanping Liu
, Qinru Sun
, Lu Yang
, Junli Dong
, Bowen Zhang
, Su Chen

医学部

Jining Medical College
Xi'an Jiaotong University
Henan University

科研成果: 期刊稿件 › 文章 › 同行评审

1 引用（Scopus）

摘要

Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).

源语言	英语
文章编号	100601
期刊	STAR Protocols
卷	2
期	2
DOI	https://doi.org/10.1016/j.xpro.2021.100601
出版状态	已出版 - 18 6月 2021

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10.1016/j.xpro.2021.100601

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title = "In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions",

abstract = "Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).",

keywords = "Cancer, Cell separation/fractionation, Molecular Biology, Protein expression and purification",

author = "Sheng Li and Guoan Zhang and Yanping Liu and Qinru Sun and Lu Yang and Junli Dong and Bowen Zhang and Su Chen",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = jun,

day = "18",

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TY - JOUR

T1 - In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions

AU - Li, Sheng

AU - Zhang, Guoan

AU - Liu, Yanping

AU - Sun, Qinru

AU - Yang, Lu

AU - Dong, Junli

AU - Zhang, Bowen

AU - Chen, Su

PY - 2021/6/18

Y1 - 2021/6/18

N2 - Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).

AB - Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).

KW - Cancer

KW - Cell separation/fractionation

KW - Molecular Biology

KW - Protein expression and purification

UR - https://www.scopus.com/pages/publications/85107822255

U2 - 10.1016/j.xpro.2021.100601

DO - 10.1016/j.xpro.2021.100601

M3 - 文章

C2 - 34169294

AN - SCOPUS:85107822255

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 2

M1 - 100601

ER -

In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions

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