TY - JOUR
T1 - IL-17A up-regulates expression of endothelial tissue factor in liver cirrhosis via the ROS/p38 signal pathway
AU - Pu, Yansong
AU - Zhang, Shu
AU - Zhou, Rui
AU - Huang, Na
AU - Li, Han
AU - Wei, Wei
AU - Li, Liang
AU - Huang, Chen
AU - Yang, Jun
AU - Li, Zongfang
N1 - Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.
PY - 2016/1/29
Y1 - 2016/1/29
N2 - Interleukin-17A (IL-17A), an inflammatory cytokine, is elevated in liver cirrhosis. Inflammation and coagulation dysfunction are closely related. Tissue factor (TF) is a bridge between endothelial activation, blood coagulation and inflammation. The aims of the present study were to evaluate endothelial TF expression in liver cirrhosis and identify the possible underlying role of IL-17A in TF expression. In the present study, we found that TF expression was increased on endothelium of splenic vein from cirrhotic patients and significantly correlated with intima/media ratios of splenic vein and coagulation parameters. Serum levels of IL-17A were significantly higher in cirrhotic patients as compared with normal controls. Cirrhotic serum and IL-17A stimulated TF expression in HUVECs, which was reduced by blockade of IL-17A, p38, and reactive oxygen species (ROS). Taken together, our data show that enhanced expression of endothelial TF, which plays an important role in coagulopathy and splenic vein remodeling in liver cirrhosis, is induced by IL-17A in a ROS dependent manner.
AB - Interleukin-17A (IL-17A), an inflammatory cytokine, is elevated in liver cirrhosis. Inflammation and coagulation dysfunction are closely related. Tissue factor (TF) is a bridge between endothelial activation, blood coagulation and inflammation. The aims of the present study were to evaluate endothelial TF expression in liver cirrhosis and identify the possible underlying role of IL-17A in TF expression. In the present study, we found that TF expression was increased on endothelium of splenic vein from cirrhotic patients and significantly correlated with intima/media ratios of splenic vein and coagulation parameters. Serum levels of IL-17A were significantly higher in cirrhotic patients as compared with normal controls. Cirrhotic serum and IL-17A stimulated TF expression in HUVECs, which was reduced by blockade of IL-17A, p38, and reactive oxygen species (ROS). Taken together, our data show that enhanced expression of endothelial TF, which plays an important role in coagulopathy and splenic vein remodeling in liver cirrhosis, is induced by IL-17A in a ROS dependent manner.
KW - Endothelial cells
KW - Interleukin-17
KW - Liver cirrhosis
KW - Reactive oxygen species
KW - Tissue factor
UR - https://www.scopus.com/pages/publications/84956578984
U2 - 10.1016/j.bbrc.2015.12.093
DO - 10.1016/j.bbrc.2015.12.093
M3 - 文章
C2 - 26742425
AN - SCOPUS:84956578984
SN - 0006-291X
VL - 470
SP - 41
EP - 47
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -