Identification of antemortem, perimortem and postmortem fractures by FTIR spectroscopy based on a rabbit tibial fracture model

The identification of antemortem, perimortem and postmortem fractures is very important for forensic pathologists and anthropologists. However, traditional methods are subjective, time-consuming, and have low accuracy, which do not fundamentally solve the problem. In this study, we utilized Fourier transform infrared (FTIR) spectroscopy and chemometrics to identify antemortem, perimortem and postmortem fractures in a rabbit tibial fracture model. Based on the results of the principal component analysis (PCA), changes in the ante-perimortem fracture repair process are mainly associated with protein variations, while postmortem fractures are more likely to result in lipid changes during degradation. Then, a partial least squares discriminant analysis (PLS-DA) was performed to assess the classification ability of the training and predictive datasets, with classification accuracies of 88.9% and 86.7%, respectively. According to the latent variable 1 (LV1) loading plot, amide I and amide II (proteins) are mostly classified as ante-perimortem and postmortem fractures. In conclusion, FTIR spectroscopy is a reliable tool to identify antemortem, perimortem and postmortem fractures. FTIR has the advantages of rapid, objective and strong discrimination. and shows great potential for analyzing forensic cases under actual natural conditions.