TY - JOUR
T1 - High-speed image reconstruction for optically sectioned, super-resolution structured illumination microscopy
AU - Wang, Zhaojun
AU - Zhao, Tianyu
AU - Hao, Huiwen
AU - Cai, Yanan
AU - Feng, Kun
AU - Yun, Xue
AU - Liang, Yansheng
AU - Wang, Shaowei
AU - Sun, Yujie
AU - Bianco, Piero R.
AU - Oh, Kwangsung
AU - Lei, Ming
N1 - Publisher Copyright:
© The Authors. Published by SPIE and CLP under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
PY - 2022/3/1
Y1 - 2022/3/1
N2 - Super-resolution structured illumination microscopy (SR-SIM) is an outstanding method for visualizing the subcellular dynamics in living cells. To date, by using elaborately designed systems and algorithms, SR-SIM can achieve rapid, optically sectioned, SR observation with hundreds to thousands of time points. However, real-time observation is still out of reach for most SIM setups as conventional algorithms for image reconstruction involve a heavy computing burden. To address this limitation, an accelerated reconstruction algorithm was developed by implementing a simplified workflow for SR-SIM, termed joint space and frequency reconstruction. This algorithm results in an 80-fold improvement in reconstruction speed relative to the widely used Wiener-SIM. Critically, the increased processing speed does not come at the expense of spatial resolution or sectioning capability, as demonstrated by live imaging of microtubule dynamics and mitochondrial tubulation.
AB - Super-resolution structured illumination microscopy (SR-SIM) is an outstanding method for visualizing the subcellular dynamics in living cells. To date, by using elaborately designed systems and algorithms, SR-SIM can achieve rapid, optically sectioned, SR observation with hundreds to thousands of time points. However, real-time observation is still out of reach for most SIM setups as conventional algorithms for image reconstruction involve a heavy computing burden. To address this limitation, an accelerated reconstruction algorithm was developed by implementing a simplified workflow for SR-SIM, termed joint space and frequency reconstruction. This algorithm results in an 80-fold improvement in reconstruction speed relative to the widely used Wiener-SIM. Critically, the increased processing speed does not come at the expense of spatial resolution or sectioning capability, as demonstrated by live imaging of microtubule dynamics and mitochondrial tubulation.
KW - high-speed image reconstruction
KW - live-cell imaging
KW - microtubule dynamics
KW - mitochondrial tubulation
KW - real-time structured illumination microscopy
UR - https://www.scopus.com/pages/publications/85129868225
U2 - 10.1117/1.AP.4.2.026003
DO - 10.1117/1.AP.4.2.026003
M3 - 文章
AN - SCOPUS:85129868225
SN - 2577-5421
VL - 4
JO - Advanced Photonics
JF - Advanced Photonics
IS - 2
M1 - 026003
ER -