High resolution melting analyzer based on high throughout fast detection of weak fluorescence

High resolution melting curves analysis (HRM) is a recently developed powerful technique for the detection of gene mutations and SNP analysis. Monitoring the changes in fluorescence from saturated dye labeled on dsDNA, as a PCR products (amplicons) are melted, the minor difference of nucleic acid sequence can be discriminated. Here fast fluorescence detection system based on fiber-optics switch array is designed for fulfilling the requirements of speed and sensitivity. High-density fluorescence data are processed by a series of procedure, such as smoothed, automatic baseline detection, normalizing and differential to temperature, different gene types could be recognized quickly and correctly by the high resolution melting curves.