Gradient decrease in telomerase and its RNA in progressive passage of human endometrial stromal stem cells

The purpose of this study was to explore the change of telomerase in passage from human endometrial stromal stem cells isolated from human endometrium. Telomerase activity of cultured endometrial stromal cells was assessed at mRNA and protein levels using RT-PCR and immunohistochemistry technique. Telomerase mRNA and protein levels were higher at early passages, and then had a gradually decreased trend of immunoreactive intensity and gradually weakened positive cells with progressive passage in endometrial stromal stem cells. These results suggest that telomerase is contributed to the insenecence of endometrial stem cell.