TY - JOUR
T1 - Ginsenoside Rd protects neurons against glutamate-induced excitotoxicity by inhibiting Ca 2+ influx
AU - Zhang, Chen
AU - Du, Fang
AU - Shi, Ming
AU - Ye, Ruidong
AU - Cheng, Haoran
AU - Han, Junliang
AU - Ma, Lei
AU - Cao, Rong
AU - Rao, Zhiren
AU - Zhao, Gang
PY - 2012/1
Y1 - 2012/1
N2 - Our previous studies have demonstrated that ginsenoside Rd (GSRd), one of the principal ingredients of Pana notoginseng, has neuroprotective effects against ischemic stroke. However, the possible mechanism(s) underlying the neuroprotection of GSRd is/are still largely unknown. In this study, we treated glutamate-injured cultured rat hippocampal neurons with different concentrations of GSRd, and then examined the changes in neuronal apoptosis and intracellular free Ca 2+ concentration. Our MTT assay showed that GSRd significantly increased the survival of neurons injured by glutamate in a dose-dependent manner. Consistently, TUNEL and Caspase-3 staining showed that GSRd attenuated glutamate-induced cell death. Furthermore, calcium imaging assay revealed that GSRd significantly attenuated the glutamate-induced increase of intracellular free Ca 2+ and also inhibited NMDA-triggered Ca 2+ influx. Thus, the present study demonstrates that GSRd protects the cultured hippocampal neurons against glutamate-induced excitotoxicity, and that this neuroprotective effect may result from the inhibitory effects of GSRd on Ca 2+ influx.
AB - Our previous studies have demonstrated that ginsenoside Rd (GSRd), one of the principal ingredients of Pana notoginseng, has neuroprotective effects against ischemic stroke. However, the possible mechanism(s) underlying the neuroprotection of GSRd is/are still largely unknown. In this study, we treated glutamate-injured cultured rat hippocampal neurons with different concentrations of GSRd, and then examined the changes in neuronal apoptosis and intracellular free Ca 2+ concentration. Our MTT assay showed that GSRd significantly increased the survival of neurons injured by glutamate in a dose-dependent manner. Consistently, TUNEL and Caspase-3 staining showed that GSRd attenuated glutamate-induced cell death. Furthermore, calcium imaging assay revealed that GSRd significantly attenuated the glutamate-induced increase of intracellular free Ca 2+ and also inhibited NMDA-triggered Ca 2+ influx. Thus, the present study demonstrates that GSRd protects the cultured hippocampal neurons against glutamate-induced excitotoxicity, and that this neuroprotective effect may result from the inhibitory effects of GSRd on Ca 2+ influx.
KW - Calcium influx
KW - Excitotoxicity
KW - Ginsenoside Rd
KW - Glutamate
KW - NMDA receptor
UR - https://www.scopus.com/pages/publications/84856531987
U2 - 10.1007/s10571-011-9742-x
DO - 10.1007/s10571-011-9742-x
M3 - 文章
C2 - 21811848
AN - SCOPUS:84856531987
SN - 0272-4340
VL - 32
SP - 121
EP - 128
JO - Cellular and Molecular Neurobiology
JF - Cellular and Molecular Neurobiology
IS - 1
ER -