@inproceedings{759ce3396f774a1c841017c597f07674,
title = "Generating long material gradients by convection and alternating flow in a microchannel",
abstract = "Natural materials exhibit anisotropy with variations in soluble factors, cell distribution, and matrix properties. The ability to recreate the heterogeneity of the natural materials is a major challenge for investigating cell-material interactions and for developing biomimetic materials. Here we present a generic fluidic approach using convection and alternating flow to rapidly generate multi-centimeter gradients of biomolecules, polymers, beads and cells and cross-gradients of two species in a microchannel. A poly(ethylene-glycol) hydrogel gradient and a porous collagen gradient were generated with continuous variations in material properties. A composite material with a gelatin/hyaluronic acid cross-gradient was generated that exhibited a continuous gradient in cell attachment. This simple yet generic fluidic platform should prove useful for creating anisotropic biomimetic materials and high-throughput platforms for investigating cell-microenvironment interaction.",
author = "Hancock, \{M. J.\} and Y. Du and J. He and Villa-Uribe, \{J. L.\} and B. Wang and Cropek, \{D. M.\} and A. Khademhosseini",
year = "2010",
doi = "10.1109/NEBC.2010.5458135",
language = "英语",
isbn = "9781424468799",
series = "Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010",
booktitle = "Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010",
note = "36th Annual Northeast Bioengineering Conference, NEBEC 2010 ; Conference date: 26-03-2010 Through 28-03-2010",
}