Fabrication and application of single nucleotide polymorphisms library on magnetic nanoparticles using adaptor PCR

Hongna Liu; Song Li; Meiju Ji; Libo Nie; Jianrong Chen; Yuqing Miao; Nongyue He

doi:10.1166/jnn.2008.014

Fabrication and application of single nucleotide polymorphisms library on magnetic nanoparticles using adaptor PCR

Hongna Liu
, Song Li
, Meiju Ji
, Libo Nie
, Jianrong Chen
, Yuqing Miao
, Nongyue He

Hunan University of Technology
Southeast University, Nanjing
Zhejiang Normal University

科研成果: 期刊稿件 › 文章 › 同行评审

64 引用（Scopus）

摘要

We have developed a novel approach to fabricate single nucleotide polymorphisms (SNPs) library on magnetic nanoparticles (MNPs) based on adaptor PCR. Each SNP locus in the library was interrogated by hybridization with a pair of allele specific dual-color fluorescence (Cy3, Cy5) probes to determine SNR Two SNPs loci (M235T and A-6G) associated with essential hypertension in the angiotensinogen (AGT) gene were detected by this method and their fluorescent signals were quantified. The fluorescent ratios (match probe: mismatch probe signal) of homozygous genotypes were over 3.0, whereas heterozygous genotypes had ratios near to 1.0. Without any complex multiplex PCR procedure, it is a simple, efficient and reliable method for the multiplex SNPs detection using limited amount of DNA samples from individuals.

源语言	英语
页（从-至）	405-409
页数	5
期刊	Journal of Nanoscience and Nanotechnology
卷	8
期	1
DOI	https://doi.org/10.1166/jnn.2008.014
出版状态	已出版 - 1月 2008
已对外发布	是

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abstract = "We have developed a novel approach to fabricate single nucleotide polymorphisms (SNPs) library on magnetic nanoparticles (MNPs) based on adaptor PCR. Each SNP locus in the library was interrogated by hybridization with a pair of allele specific dual-color fluorescence (Cy3, Cy5) probes to determine SNR Two SNPs loci (M235T and A-6G) associated with essential hypertension in the angiotensinogen (AGT) gene were detected by this method and their fluorescent signals were quantified. The fluorescent ratios (match probe: mismatch probe signal) of homozygous genotypes were over 3.0, whereas heterozygous genotypes had ratios near to 1.0. Without any complex multiplex PCR procedure, it is a simple, efficient and reliable method for the multiplex SNPs detection using limited amount of DNA samples from individuals.",

keywords = "Adaptor PCR, Magnetic nanoparticles, Microarray, Single nucleotide polymorphisms",

author = "Hongna Liu and Song Li and Meiju Ji and Libo Nie and Jianrong Chen and Yuqing Miao and Nongyue He",

year = "2008",

month = jan,

doi = "10.1166/jnn.2008.014",

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AU - Liu, Hongna

AU - Li, Song

AU - Ji, Meiju

AU - Nie, Libo

AU - Chen, Jianrong

AU - Miao, Yuqing

AU - He, Nongyue

PY - 2008/1

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N2 - We have developed a novel approach to fabricate single nucleotide polymorphisms (SNPs) library on magnetic nanoparticles (MNPs) based on adaptor PCR. Each SNP locus in the library was interrogated by hybridization with a pair of allele specific dual-color fluorescence (Cy3, Cy5) probes to determine SNR Two SNPs loci (M235T and A-6G) associated with essential hypertension in the angiotensinogen (AGT) gene were detected by this method and their fluorescent signals were quantified. The fluorescent ratios (match probe: mismatch probe signal) of homozygous genotypes were over 3.0, whereas heterozygous genotypes had ratios near to 1.0. Without any complex multiplex PCR procedure, it is a simple, efficient and reliable method for the multiplex SNPs detection using limited amount of DNA samples from individuals.

AB - We have developed a novel approach to fabricate single nucleotide polymorphisms (SNPs) library on magnetic nanoparticles (MNPs) based on adaptor PCR. Each SNP locus in the library was interrogated by hybridization with a pair of allele specific dual-color fluorescence (Cy3, Cy5) probes to determine SNR Two SNPs loci (M235T and A-6G) associated with essential hypertension in the angiotensinogen (AGT) gene were detected by this method and their fluorescent signals were quantified. The fluorescent ratios (match probe: mismatch probe signal) of homozygous genotypes were over 3.0, whereas heterozygous genotypes had ratios near to 1.0. Without any complex multiplex PCR procedure, it is a simple, efficient and reliable method for the multiplex SNPs detection using limited amount of DNA samples from individuals.

KW - Adaptor PCR

KW - Magnetic nanoparticles

KW - Microarray

KW - Single nucleotide polymorphisms

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DO - 10.1166/jnn.2008.014

M3 - 文章

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JO - Journal of Nanoscience and Nanotechnology

JF - Journal of Nanoscience and Nanotechnology

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Fabrication and application of single nucleotide polymorphisms library on magnetic nanoparticles using adaptor PCR

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