Expression of H19 imprinted genes in the villus tissues of women with spontaneous abortion

Objective: To investigate the expression of HI9 imprinted genes in the villus tissues of women with spontaneous abortion. Methods: Reverse transcription-polymerse chain reaction method(RT-PCR) was employed to detect H19 imprinted genes allele-specific expression levels of the villus tissues extracted from 45 cases of spontaneous abortion and 30 cases of normal pregnancy. Results: Nineteen cases of 21 heterozygous cases in spontaneous abortion samples showed billelic expression of H19 (19/21), whereas no billelic expression was found in the normal pregnancy samples (0/13) (P<0.05). Conclusion: Significant imprint loss of H19 gene in villus tissues of women with spontaneous abortion may play some role in the pathogenesis of spontaneous abortion.