Enhanced in-vitro osteoblastic functions on β-type titanium alloy using surface mechanical attrition treatment

To improve the osseointegration of titanium based implants, we herein modified Ti-25Nb-3Mo-2Sn-3Zr, a β-type titanium alloy by means of surface mechanical attrition treatment (SMAT). X-ray diffraction and transmission electron microscope results jointly indicate that SMAT process refined the average grain size of β phase in the surface layer of the alloy from about 110 μm to 26 nm. Besides, the surface properties and in-vitro cell culture tests of the SMAT-processed samples were investigated compared to those on the non-treated samples. Atomic force microscope and hydrophilicity tests revealed that the SMAT-processed surface was much rougher and hydrophilic than the non-treated surface. In vitro experimental results showed that SMAT-processed surface promoted adsorption of total protein as well as anchoring proteins such as vitronectin and fibronectin on its surface from cell culture medium, furthermore, significant improvements of osteoblast adhesion, proliferation, differentiation and extracellular mineralization were also found on the SMAT-processed surface compared to the non-treated surface. This could be attributed to the grain refinement as well as increased surface hydrophilicity and roughness after SMAT process. Our study provides a promising means of surface modification for future use in biomedical application.