TY - JOUR
T1 - Electrohydrodynamic-Printed Dual-Triphase Microfibrous Scaffolds Reshaping the Lipidomic Profile for Enthesis Healing in a Rat Rotator Cuff Repair Model
AU - Bai, Lang
AU - Kasimu, Ayiguli
AU - Wang, Shuai
AU - Qiu, Zhennan
AU - Xu, Meiguang
AU - Qu, Xiaoli
AU - Chen, Baojun
AU - Liu, Qiaonan
AU - Ai, Yixiang
AU - Li, Meng
AU - Xiu, Jintao
AU - Liu, Kai
AU - Wen, Nuanyang
AU - He, Jiankang
AU - Zhang, Jing
AU - Yin, Zhanhai
N1 - Publisher Copyright:
© 2024 Wiley-VCH GmbH.
PY - 2025/1/15
Y1 - 2025/1/15
N2 - Rotator cuff injuries often result in chronic pain and functional limitations due to retears and scar formation at the enthesis. This study assess the efficacy of electrohydrodynamic-printed microfibrous dual-triphase scaffolds (DTSs), designed to optimize enthesis repair. These scaffolds, composed of polycaprolactone enhanced with nanohydroxyapatite, nano-magnesium-oxide, and kartogenin demonstrate significant biological advantages. In vitro, the scaffolds support over 95% stem cell viability and promote enhanced expression of critical markers such as tenomodulin (TNMD), sex-determining region Y-Box transcription factor 9 (SOX-9), and runt-related transcription factor 2 (RUNX-2). Enhanced expressions of tendon markers tenomodulin and scleraxis (SCX) are noted, alongside significant upregulation of chondrocyte and osteoblast markers. In vivo, these scaffolds significantly improve the biomechanical properties of the repaired enthesis, with a maximum failure load of 27.0 ± 4.2 N and ultimate stress of 5.5 ± 1.0 MPa at 6 weeks postimplantation. Lipidomic analysis indicates substantial regulation of phospholipids such as phosphatidylcholine and phosphatidylserine, highlighting the scaffold's capacity to modulate biochemical pathways critical for tissue repair and regeneration. This study underscores the potential of DTS to improve clinical outcomes in rotator cuff injury treatment by enhancing cellular differentiation, biomechanical properties, and biochemical environment, setting a foundation for personalized treatment strategies in tendon–bone repair.
AB - Rotator cuff injuries often result in chronic pain and functional limitations due to retears and scar formation at the enthesis. This study assess the efficacy of electrohydrodynamic-printed microfibrous dual-triphase scaffolds (DTSs), designed to optimize enthesis repair. These scaffolds, composed of polycaprolactone enhanced with nanohydroxyapatite, nano-magnesium-oxide, and kartogenin demonstrate significant biological advantages. In vitro, the scaffolds support over 95% stem cell viability and promote enhanced expression of critical markers such as tenomodulin (TNMD), sex-determining region Y-Box transcription factor 9 (SOX-9), and runt-related transcription factor 2 (RUNX-2). Enhanced expressions of tendon markers tenomodulin and scleraxis (SCX) are noted, alongside significant upregulation of chondrocyte and osteoblast markers. In vivo, these scaffolds significantly improve the biomechanical properties of the repaired enthesis, with a maximum failure load of 27.0 ± 4.2 N and ultimate stress of 5.5 ± 1.0 MPa at 6 weeks postimplantation. Lipidomic analysis indicates substantial regulation of phospholipids such as phosphatidylcholine and phosphatidylserine, highlighting the scaffold's capacity to modulate biochemical pathways critical for tissue repair and regeneration. This study underscores the potential of DTS to improve clinical outcomes in rotator cuff injury treatment by enhancing cellular differentiation, biomechanical properties, and biochemical environment, setting a foundation for personalized treatment strategies in tendon–bone repair.
KW - dual-triphase structure
KW - enthesis
KW - fibrocartilage regeneration
KW - lipidomics
KW - rotator cuff repair
KW - scaffold
KW - tissue engineering
UR - https://www.scopus.com/pages/publications/85210002348
U2 - 10.1002/smll.202406069
DO - 10.1002/smll.202406069
M3 - 文章
C2 - 39580676
AN - SCOPUS:85210002348
SN - 1613-6810
VL - 21
JO - Small
JF - Small
IS - 2
M1 - 2406069
ER -