Effects of TGF-β1 on the migration, adhesion and proliferation of periodontal ligament stem cells

Objective: To evaluate the effects of transforming growth factor β1(TGF-β1) on migration, adhesion and proliferation of periodontal ligament stem cells (PDLSCs) and explore the mechanisms of PDLSCs-induced periodontal remodeling. Methods: PDLSCs were isolated and identified from human teeth. The effect of TGF-β1 on migration of PDLSCs was evaluated using transwell migration assay. Cells attachment assay was used to test the effect of TGF-β1 on the adhesion of PDLSCs. In addition, the effect of TGF-β1 on the proliferation of PDLSCs was evaluated by MTT and cell growth rate assay. Results: The results showed that TGF-β1 induced the migration of PDLSCs in a dose-dependent manner, improved the adhesion and proliferation of PDLSCs. So we propose that TGF-β1 may promote periodium remodeling by inducing PDLSCs migration, following adhesion and proliferation in these areas. Conclusion: This study demonstrated for the first time that TGF-β1 increases the adhesion and migration of PDLSCs in vitro. The signal pathway is involved in the TGF-β1-induced migration of PDLSCs and the mechanical-chemical interaction during the orthodontic periodontal remodeling will be researched in our further studies.