Effects of Sp2 on the proliferation of cervical cancer Hela cells and expression of Cyclin D1

Objective: To investigate the effects of transcription factor (Sp2) on the proliferation of cervical cancer Hela cells and the molecular mechanisms.

Methods: The expression of Sp2 in cervical cancer tissues and effects of Sp2 siRNA on the expressions of Sp2 and Cyclin D1 RNA and protein were analyzed by Real-time PCR and Western Blot, respectively. MTT assay was used to analyze the effects of Sp2 on the proliferation of cervical cancer Hela cells. The effects of Sp2 on cell cycle and apoptosis of cervical cancer Hela cells were analyzed with flow cytometer.

Results: Sp2 expression level was higher in human cervical cancer tissues than in normal cervical tissues. Sp2 siRNA inhibited the proliferation of cervical cancer Hela cells. The number of S and G2/M phase cells significantly decreased in Sp2 siRNA group compared with the control group (P<0.01). Meanwhile, the number of G1/G0 phase cells increased remarkably (P<0.01). It was found that Sp2 and Cyclin D1 expressions at the mRNA and protein levels downregulated significantly in Sp2 siRNA group compared with the control group (P<0.01).

Conclusion: Sp2 promotes the proliferation of cervical cancer Hela cells through up-regulating Cyclin D1 expression.