Effects of NaF and H₂O₂ on expression of vascular endothelial growth factor in fibroblasts of rats

Objective: To compare the effects of different doses of sodium fluoride (NaF) and hydrogen peroxide (H₂O₂) on expression of vascular endothelial growth factor(VEGF) in fibroblasts of rats, and to explore a simple and effective way to promote the expression of VEGF, and in order to provide some theoretical reference to optimize the dermis culture condition in tissue engineering. Methods: The rat fibroblasts were isolated by enzyme digestion, and they were cultivated respectively with DMEM medium contained different doses of sodium fluoride (0.05 and 0.50 mmol · L ^-1)and H₂O₂ (10 nmol · L^-1 and 10 nmol · L^-1), or with both two factors(NaF 0.05 mmol · L^-1, H₂O₂ 10 μmol · L ^-1). The proliferation ability was evaluated by CCK-8 assay at 0, 24 and 48 h after the fibroblasts were treated with these factors. And the effects of NaF and H₂O₂ on the expression of VEGF were evaluated by ELISA assay and immunohistochemical method at 48 h. Results: There was no evidently inhibitory effects of different doses of NaF and H₂O ₂ used alone or combined on the proliferation of fibroblas, there was no significant difference compared with blank control (P>0.05). The expressions of VEGF in both NaF group and H₂O₂ group were greatly improved, there was significant difference compared with blank control group(P<0.05). However, the expression of VEGF in the fibroblasts treated with combined two factors was most enhanced, which could increase 40%(P<0.05). Conclusion: The combination of NaF and H²O ² can keep the normal proliferation activity of fibroblasts, and promote the expression of VEGF effectively.