Effects of antimony on anaerobic methane oxidization and microbial community in an antimony-contaminated paddy soil: A microcosm study

Anaerobic methane oxidation (AOM) plays an important role in sinking global methane and thereby in constraining climate change. Anthropogenic activities in antimony (Sb) mines have resulted in Sb contamination in rice fields, which are among the dominant methane sources. However, the knowledge of effects of Sb on AOM in paddy soils and the microbiota involved in this process remains limited. Herein, Sb was amended into the paddy soil to investigate the effects of Sb contamination on AOM and the microbial communities such as methanotrophs. Significant inhibition of AOM was observed in the treatment with Sb addition in comparison with the treatment without Sb addition. The significant increases in the abundance of the mcrA genes, responsible for methane production and oxidation, were observed in the treatment with/without Sb addition. In contrast, no significant increases in the copy number of the mcrA gene were detected in the treatment with the addition of the methanogenic and methanotrophic inhibitor 2-bromoethanesulfonate (BES). These results suggested that Sb contamination might inhibit only AOM but not methane production. In addition, amplicon high-throughput sequencing showed that the Sb addition impaired the diversity of microbial communities and impacted the biotic interactions in the soil. However, the abundance of bacterial methane-oxidizing phylum NC10 and its biotic connections with other microbes were enhanced by the addition of Sb. Pseudogulbenkiania and Methanosarcina dominated the bacterial and archaeal communities in the treatment without Sb addition, while the bacteria Ramlibacter and Geothrix and the archaea Methanomethylovorans were the most abundant genera in the treatment with Sb addition. These analyses of microbial communities indicated that Sb addition had significant effects on both the compositions of bacterial and archaeal communities. This study expands our knowledge of the effects of Sb contamination on AOM and the microbial (especially methanotrophs) diversity and composition in paddy soils.