Effect and mechanism of Danhong injection on isolated mesenteric arterial rings in rats

Objective: To study the effect and mechanism of Danhong injection on isolated mesenteric arterial rings in rats. Method: An isolated vascular ring experiment was conduced to determine the changes in tension of vascular rings with a biological signal collection and analytical system. Result: Danhong injection had no impact on the tension of vascular rings. Danhong injection showed a significant vasodilatation effect on treated arteria rings of norepinephrine, and no remarkable impact was made on the effect without endothium. It showed notable effect on blood vessels treated with Ca ²⁺ and no significant impact on those treated with caffeine. It could inhibit NE-induced intracellular calcium from releasing and external calcium from inflowing. No effects of potassium channel blockers on aorta ring tensile force were found. Conclusion: Danhong injection shows significant vasodilation effect, which mainly works through vascular smooth muscle. Its vasodilation effect may be related to inhibitory receptor, voltage-dependent Ca ²-release and IP₃ receptor-mediated Ca²⁺- influx.