TY - GEN
T1 - Dual extracellular recording using a light-addressable potentiometric sensor for taste signal transduction
AU - Wu, Chunsheng
AU - Du, Liping
AU - Qin, Zhen
AU - Gao, Keqiang
AU - Wang, Ping
N1 - Publisher Copyright:
© 2017 IEEE.
PY - 2017/7/5
Y1 - 2017/7/5
N2 - Mammalian taste system can detect and distinguish various taste signals presented by different tastants using various kinds of taste bud cells. This study described a novel bioelectronic sensor in which taste bud cells were used as biotransducer for detecting bitter signals to investigate taste signal transduction. Using a light-addressable potentiometric sensor (LAPS) as sensing chip to monitor both extracellular potential and ATP secretion of the taste bud cells, we develop the structure and analyzed the feasibility of taste bud cell-based biosensor used for studing taste signal transduction. We test the performance of this hybrid biosensor by using bitter taste substances, denatonium, as stimulations to taste bud cells. The results show that this hybrid biosensor is sensitive to cell membrane potential changes and ATP secretion under specific taste stimulations, which could help to reveal the relationship between cell membrane potential changes and ATP secretion of taste bud cells. All these results suggest this hybrid cell-based biosensor could be a potential tool for studing taste signal transduction.
AB - Mammalian taste system can detect and distinguish various taste signals presented by different tastants using various kinds of taste bud cells. This study described a novel bioelectronic sensor in which taste bud cells were used as biotransducer for detecting bitter signals to investigate taste signal transduction. Using a light-addressable potentiometric sensor (LAPS) as sensing chip to monitor both extracellular potential and ATP secretion of the taste bud cells, we develop the structure and analyzed the feasibility of taste bud cell-based biosensor used for studing taste signal transduction. We test the performance of this hybrid biosensor by using bitter taste substances, denatonium, as stimulations to taste bud cells. The results show that this hybrid biosensor is sensitive to cell membrane potential changes and ATP secretion under specific taste stimulations, which could help to reveal the relationship between cell membrane potential changes and ATP secretion of taste bud cells. All these results suggest this hybrid cell-based biosensor could be a potential tool for studing taste signal transduction.
KW - Adenosine triphosphate (ATP)
KW - Biosensor
KW - Extracellular recording
KW - Light-addressable potentiometric sensor (LAPS)
KW - Taste bud cell
UR - https://www.scopus.com/pages/publications/85027225413
U2 - 10.1109/ISOEN.2017.7968920
DO - 10.1109/ISOEN.2017.7968920
M3 - 会议稿件
AN - SCOPUS:85027225413
T3 - ISOEN 2017 - ISOCS/IEEE International Symposium on Olfaction and Electronic Nose, Proceedings
BT - ISOEN 2017 - ISOCS/IEEE International Symposium on Olfaction and Electronic Nose, Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2017 ISOCS/IEEE International Symposium on Olfaction and Electronic Nose, ISOEN 2017
Y2 - 28 May 2017 through 31 May 2017
ER -